D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse SSEA-1 PE-conjugated Monoclonal Antibody (Catalog # FAB2155P, filled histogram) or isotype control antibody (IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of SSEA-1 in Whole blood granulocytes cells by Flow Cytometry.

Whole blood granulocytes cells were stained with Mouse Anti-Human/Mouse SSEA-1 PE-conjugated Monoclonal Antibody (Catalog # FAB2155P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse SSEA-1 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µL/10⁶ cells
Sample: D3 mouse embryonic stem cell line and Whole blood granulocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

References

Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
Fox, N. et al. (1983) Cancer Res. 43:669.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Additional SSEA-1 Products

Product Documents for Human/Mouse SSEA-1 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse SSEA-1 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse SSEA-1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2155P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all SSEA-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse SSEA-1 PE-conjugated Antibody

Detection of SSEA‑1 in D3 Mouse Cell Line by Flow Cytometry.