Human/Mouse SSEA-4 Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1435F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Product Specifications
Immunogen
2120Ep human embryonal carcinoma cell line
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human/Mouse SSEA-4 Fluorescein-conjugated Antibody
Detection of SSEA‑4 in NTera‑2 human testicular embryonic carcinoma cell line by Flow Cytometry.
NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1435F, filled histogram) or isotype control antibody (Catalog # IC007F, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human/Mouse SSEA-4 Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line
Sample: NTera‑2 human testicular embryonic carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SSEA-4
SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).
References
- Shevinsky, L.H. et al. (1982) Cell 30:697.
- Kannagi, R. et al. (1983) EMBO J. 2:2355.
- Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
- Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4
Additional SSEA-4 Products
Product Documents for Human/Mouse SSEA-4 Fluorescein-conjugated Antibody
Product Specific Notices for Human/Mouse SSEA-4 Fluorescein-conjugated Antibody
For research use only
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