Human/Mouse TANK Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4755
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse TANK
Ala157-Thr448
Accession # P70347
Ala157-Thr448
Accession # P70347
Specificity
Detects endogenous human and mouse TANK in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse TANK Antibody
Detection of Human/Mouse TANK by Western Blot.
Western blot shows lysates of A20 mouse B cell lymphoma cell line, DA3 mouse myeloma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse TANK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TANK at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse TANK Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: A20 mouse B cell lymphoma cell line, DA3 mouse myeloma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line
Sample: A20 mouse B cell lymphoma cell line, DA3 mouse myeloma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TANK
TRAF (tumor necrosis factor receptor-associated factor) family member-associated NF-kappaB activator (TANK) maintains TRAF1, TRAF2 and TRAF3 proteins in their latent states (cytoplasmic sequestration). TANK binds to their TRAF-c domains inhibiting TRAF signaling and NF kappaB activation. TANK may function as an adaptor molecule in multiple antiviral pathways. Two transcript variants encoding different isoforms for this gene have been found.
Long Name
TRAF Family Member-Associated NFKB Activator
Alternate Names
I-TRAF
Gene Symbol
TANK
UniProt
Additional TANK Products
Product Documents for Human/Mouse TANK Antibody
Product Specific Notices for Human/Mouse TANK Antibody
For research use only
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