Human/Mouse TREM2 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB17291P

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 237920

View all TREM2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREM-2
extracellular domain
Accession # Q99NH8

Specificity

Detects human and mouse TREM-2 in direct ELISAs and Western blots. Stains TREM-2 transfectants but not TREM-1 transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse TREM2 PE-conjugated Antibody

Detection of TREM-2 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of TREM‑2 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM-2 PE-conjugated Monoclonal Antibody (Catalog # FAB17291P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of TREM-2 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of TREM‑2 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Rat Anti-Human/Mouse TREM-2 PE-conjugated Monoclonal Antibody (Catalog # FAB17291P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of TREM-2 antibody in THP-1 Human Cell Line antibody by Flow Cytometry.

Detection of TREM‑2 in THP‑1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Rat Anti-Human/Mouse TREM-2 PE-conjugated Monoclonal Antibody (Catalog # FAB17291P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Applications for Human/Mouse TREM2 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line, human peripheral blood monocytes, THP‑1 human acute monocytic leukemia cell line, human mature dendritic cells, and HEK293 human embryonic kidney cell line transfected with eGFP and either human TREM-2 or human TREM-1

Reviewed Applications

Read 1 review rated 5 using FAB17291P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Protect from light.

Background: TREM2

TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily . Mature human TREM-2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM-2 shares 73% and 74% aa sequence identity with mouse and rat TREM-2, respectively. Two closely related transcripts were reported in mouse and designated TREM-2a and TREM-2b. Soluble forms of the TREM-2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM-2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM-2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM-2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM-2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM-2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM-2 blockade exacerbates disease symptoms in the experimental EAE model of MS.

Long Name

Triggering Receptor Expressed on Myeloid Cells 2

Alternate Names

PLOSL2, TREM-2

Entrez Gene IDs

54209 (Human); 102133279 (Cynomolgus Monkey)

Gene Symbol

TREM2

UniProt

Q99NH8 (Mouse)

Additional TREM2 Products

Product Documents for Human/Mouse TREM2 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse TREM2 PE-conjugated Antibody

For research use only

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