Human/Mouse UCP1 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC6158P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Xenograft
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 536435
Product Specifications
Immunogen
E. coli-derived recombinant human UCP1
Met1-Thr307
Accession # P25874
Met1-Thr307
Accession # P25874
Specificity
Detects human and mouse UCP1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse UCP1 PE-conjugated Antibody
Detection of UCP1 in Human Preadipocytes by Flow Cytometry.
Human preadipocytes were stained with Mouse Anti-Human/Mouse UCP1 PE-conjugated Monoclonal Antibody (Catalog # IC6158P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of UCP1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.
3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 PE-conjugated Monoclonal Antibody (Catalog # IC6158P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse UCP1 PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human preadipocytes and 3T3‑L1 mouse embryonic fibroblast adipose-like cell line
Sample: Human preadipocytes and 3T3‑L1 mouse embryonic fibroblast adipose-like cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied
in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: UCP1
Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.
Long Name
Uncoupling Protein 1
Alternate Names
SLC25A7, Thermogenin
Gene Symbol
UCP1
UniProt
Additional UCP1 Products
Product Documents for Human/Mouse UCP1 PE-conjugated Antibody
Product Specific Notices for Human/Mouse UCP1 PE-conjugated Antibody
For research use only
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