Human Mrc2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5770

R&D Systems, part of Bio-Techne
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MAB5770-100
MAB5770-SP
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1063704

View all Mrc2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived human Mrc2
Gly31-Ala1414
Accession # Q9UBG0

Specificity

Detects human Mrc2 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Mrc2 Antibody

Detection of Mrc2 in THP-1 cells (positive) and A431 cells (negative).

Mrc2 was detected in immersion fixed THP-1 human acute monocytic leukemia cells (positive) and absent in A431 human epithelial carcinoma cells (negative) using Mouse Anti-Human Mrc2 Monoclonal Antibody (Catalog # MAB5770) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mrc2 in THP-1 cells by Flow Cytometry.

THP-1 cells were stained with Mouse Anti-Human Mrc2 Monoclonal Antibody (Catalog # MAB5770, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Mrc2 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: THP-1 human acute monocytic leukemia cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed THP-1 human acute monocytic leukemia cells (positive) and A431 human epithelial carcinoma cells (negative)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mrc2

Mrc2 (C-type Mannose Receptor 2),  also know as MMR2, Endocytic Receptor 180 and uPARAP,  is a 180‑kDa type I transmembrane protein. It is one of the mannose receptor (MR) family members which share a common domain organization and have a broad range of biological functions (1). Mrc2 is an endocytic receptor that is found on migrating cells, including cancer cells, macrophages, fibroblasts and endothelial cells (2). Mature human Mrc2 is composed of 1449 amino acid (aa) that includes a 1384 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 44 aa cytoplasmic domain. The ECD shows one ricin B‑type lectin domain, one fibronectin type II domain and eight C‑type lectin domains. Within the ECD, human Mrc2 shares 91% aa identity with mouse and rat Mrc2.  Mrc2 plays an important role in extracellular matrix remodeling through interaction with its ligands, including Man, Fuc, NAcGlc, collagens and urokinase plasminogen activator receptor (uPAR) (1-3). This cell surface molecule has been reported to promote cell invasion through matrix remodeling by internalizing large fragments of collagen and routing it to the lysosome for intracellular degradation and cell chemotaxis (2). It has also been reported to interact with matrix metalloprotease-13 (MMP-13) and collagen V on the cell surface (4).

References

  1. Yuan, C. et al. (2016) Biochem. J. 473:2359.
  2. Durrel, T. et al. (2018) Nat. Commun. 9:5178.
  3. Behrendt, N. et al. (2000) J. Biol. Chem. 275:1993.
  4. Englehom, L.H. et al. (2001) Lab. Invest. 81:1403.

Long Name

Mannose Receptor, C-type 2

Alternate Names

CD280, CLEC13E, Endo180, uPARAP

Entrez Gene IDs

9902 (Human); 17534 (Mouse); 498011 (Rat)

Gene Symbol

MRC2

UniProt

Q9UBG0

Additional Mrc2 Products

Product Documents for Human Mrc2 Antibody

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Product Specific Notices for Human Mrc2 Antibody

For research use only

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