Human MRP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB19291
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # QCRL
Product Specifications
Immunogen
H69AR human small cell lung cancer cell line membrane extracts
Specificity
Detects human MRP1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MRP1 Antibody
MRP1 in Human PBMCs.
MRP1 was detected in immersion fixed Human peripheral blood mononuclear cells using Mouse Anti-Human MRP1 Monoclonal Antibody (Catalog # MAB19291) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human MRP1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Human peripheral blood mononuclear cells
Sample: Immersion fixed Human peripheral blood mononuclear cells
Immunoprecipitation
Hipfner, D.R., et al. (1994) Canc. Res. 54:5788.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: H69AR human small cell lung cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: H69AR human small cell lung cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
Hipfner, D.R. et al. (1994) Canc. Res. 54:5788; Hipfner, D.R. et al. (1996) Canc. Res. 56:3307. This application was not tested by R&D Systems.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB19291 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MRP1
MRP1 (Multidrug Resistance associated Protein 1), also known as ABCC1, belongs to the family of ATP-Binding Cassette (ABC) transporters. It confers multidrug resistance on tumor cells and is overexpressed in various human multidrug resistant tumor cell lines.
Long Name
Multi-drug Resistance Protein 1
Alternate Names
ABCC1, GS-X
Gene Symbol
ABCC1
Additional MRP1 Products
Product Documents for Human MRP1 Antibody
Product Specific Notices for Human MRP1 Antibody
For research use only
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