Human MYH6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8979
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 940344
Product Specifications
Immunogen
Human MYH6 peptide
Accession # P13533
Accession # P13533
Specificity
Detects human MYH6. The peptide immunogen sequence represents a region specific to MYH6 and
not present in other MYH isoforms.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MYH6 Antibody
MYH6 in Human Heart.
MYH6 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MYH6 in BG01V Human Embryonic Stem Cells.
MYH6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human MYH6 by Western Blot.
Western blot shows lysates of human heart atrium. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MYH6 Monoclonal Antibody (Catalog # MAB8979) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MYH6 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human MYH6 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart
Sample: Immersion fixed paraffin-embedded sections of human heart
Western Blot
10 µg/mL
Sample: Human heart (atrium) tissue
Sample: Human heart (atrium) tissue
Reviewed Applications
Read 2 reviews rated 4.5 using MAB8979 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MYH6
Long Name
Myosin Heavy Chain 6, Cardiac Muscle, Alpha
Alternate Names
alpha-MHC, ASD3, CMD1EE, CMH14, MyHC-Alpha, MYHCA, Myosin-6, SSS3
Gene Symbol
MYH6
UniProt
Additional MYH6 Products
Product Documents for Human MYH6 Antibody
Product Specific Notices for Human MYH6 Antibody
For research use only
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