Human Myogenin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB66861
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 671037
Product Specifications
Immunogen
E. coli-derived recombinant human Myogenin
Ser124-Asn224
Accession # P15173
Ser124-Asn224
Accession # P15173
Specificity
Detects human Myogenin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Myogenin Antibody
Detection of Human Myogenin by Western Blot.
Western blot shows lysates of Rh30 human rhabdomyosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myogenin at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Myogenin in C2C12 Mouse Cell Line.
Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cell line using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Myogenin Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed differentiated C2C12 mouse myoblast cell line
Sample: Immersion fixed differentiated C2C12 mouse myoblast cell line
Western Blot
1 µg/mL
Sample: Rh30 human rhabdomyosarcoma cell line
Sample: Rh30 human rhabdomyosarcoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB66861 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myogenin
Alternate Names
BHLHC3, MYF4, MYOG
Gene Symbol
MYOG
UniProt
Additional Myogenin Products
Product Documents for Human Myogenin Antibody
Product Specific Notices for Human Myogenin Antibody
For research use only
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