Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5469
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 706824
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human N-Acetylglucosaminyltransferase V/MGAT5
Leu189-Leu741
Accession # Q09328
Leu189-Leu741
Accession # Q09328
Specificity
Detects human N-Acetylglucosaminyltransferase V/MGAT5 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
Detection of Human N‑Acetylglucosaminyltransferase V/MGAT5 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for N-Acetylglucosaminyltransferase V/MGAT5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
N‑Acetylglucosaminyltransferase V/MGAT5 in MCF‑7 Human Cell Line.
N-Acetylglucosaminyltransferase V/MGAT5 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Reviewed Applications
Read 1 review rated 4 using MAB5469 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: N-Acetylglucosaminyltransferase V/MGAT5
N-Acetylglucosaminyltransferase V (GnT-V), also known as mannosylglycoprotein N-acetyl-glucosaminyltransferase 5 (MGAT5), adds an N-acetylglucosamine to the alpha1-6-linked core mannose of an N-linked oligosaccharide in the Golgi apparatus (1). This reaction is the committing step for the biosynthesis of beta1-6GlcNAc-branched arm in N-glycans. The degree of N-glycan branching has been shown to regulate cell proliferation and differentiation (2). An increase in the GnT-V activity and its glycan products is also known to positively correlate with the progression of invasive malignancies (3, 4). For example, ectopic expression of GnT-V in epithelial cells results in morphological transformation and tumor growth in mice and overexpression in carcinoma cells has been shown to induce metastatic spread (3-5).
References
- Saito, H. et al. (1994) Biochem. Biophys. Res. Commun. 198:318.
- Lau, K.S. et al. (2007) Cell 198:123.
- Dennis, J.W. et al. (2002) Biochim. Biophys. Acta 1573:414.
- Granovsky, M. et al. (2008) Nat. Med. 7:1.
- Kim, Y.S. et al. (2008) Mol. Cell. Proteomics 7:1.
Long Name
alpha-1,6-Mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Alternate Names
MGAT5, N-Acetylglucosaminyltransferase V
Gene Symbol
MGAT5
UniProt
Additional N-Acetylglucosaminyltransferase V/MGAT5 Products
Product Documents for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
Product Specific Notices for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
For research use only
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