Human NDRG1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5209
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NDRG1
Met111-Asp267
Accession # Q92597
Met111-Asp267
Accession # Q92597
Specificity
Detects human NDRG1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human N-myc is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human NDRG1 Antibody
Detection of Human NDRG1 by Western Blot.
Western blot shows lysates of human liver tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NDRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5209) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NDRG1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human NDRG1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human liver tissue and HeLa human cervical epithelial carcinoma cell line
Sample: Human liver tissue and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NDRG1
NDRG1 (N-myc downstream-regulated gene 1; also DRG-1 and Cap43) is a 43 kDa member of the NDRG family of proteins. It is ubiquitously expressed, found in fibroblasts, endothelial cells, respiratory epithelium and prostate epithelium. NDRG1 is highly expressed in nonproliferating, differentiating tissue. Human NDRG1 is 394 amino acids (aa) in length. It contains an NDGR domain (aa 286‑316) plus three tandem 10 aa hydrophilic repeats (aa 339‑368). There are three potential isoform variants. Two involve alternate start sites at Met82 and Met286, while a third shows a four aa substitution for aa 1‑34. Over aa 11‑267, human NDRG1 is 95% aa identical to mouse NDRG1.
Long Name
N-myc Downstream Regulated Gene 1
Alternate Names
CAP43, CMT4D, DRG1, GC4, HMSNL, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5
Gene Symbol
NDRG1
UniProt
Additional NDRG1 Products
Product Documents for Human NDRG1 Antibody
Product Specific Notices for Human NDRG1 Antibody
For research use only
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