Human Nephrin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB42692
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Capture
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1037720
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived human Nephrin
Gln23-Thr1029
Accession # O60500
Gln23-Thr1029
Accession # O60500
Specificity
Detects human Nephrin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Nephrin Antibody
Detection of Human Nephrin by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line and human kidney (medulla). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Nephrin Monoclonal Antibody (Catalog # MAB42692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Nephrin at approximately 134 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human Nephrin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEK293T human embryonic kidney cell line and human kidney (medulla)
Sample: HEK293T human embryonic kidney cell line and human kidney (medulla)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nephrin
Nephrin (or renal glomerulus-specific cell adhesion receptor; also NPHS1) is a 185 - 200 kDa member of the Ig superfamily. It is expressed on glomerular podocyte membranes, associates with same-cell Neph1, and forms homomeric zipper-like complexes with Nephrin on adjacent podocytes. Mature human Nephrin is a type I transmembrane glycoprotein 1219 amino acids (aa) in length. It contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential, soluble splice form is known where aa’s 1056 - 1095 are deleted, eliminating the transmembrane region. Over aa 23 - 1029, human Nephrin shares 84% and 89% aa identity with mouse and dog Nephrin, respectively.
Alternate Names
CNF, NPHS1
Gene Symbol
NPHS1
UniProt
Additional Nephrin Products
Product Documents for Human Nephrin Antibody
Product Specific Notices for Human Nephrin Antibody
For research use only
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