Western blot shows lysates of human uterus tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Nope at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Nope/IGDCC4 in HEK293 Human Cell Line Transfected with Human NOPE and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with either (A) human Nope or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Detection of Nope/IGDCC4 in A549 Human Cell Line by Flow Cytometry.

A549 human cell line was stained with Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345, filled histogram) or Rabbit IgG control antibody (Catalog # MAB1050, open histogram) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

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Applications for Human Nope/IGDCC4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Nope and eGFP and A549 Human Cell Line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta tissue

Western Blot

1 µg/mL
Sample: Human Uterus Tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nope/IGDCC4

IGDCC4/Nope is a type I transmembrane protein that belongs to the immunoglobulin superfamily. It is expressed mostly in embryonic muscle tissues and in developing and adult nervous systems. The structural similarities between IGDCC4 and the guidance receptor of the DCC family suggest that IGDCC4 may have similar functions as the DCC family.

Long Name

Neighbor of Punc E11/Immunoglobulin Superfamily, DCC Subclass, Member 4

Alternate Names

DDM36, IGDCC4

Entrez Gene IDs

57722 (Human); 56741 (Mouse)

Gene Symbol

IGDCC4

UniProt

Q8TDY8

Additional Nope/IGDCC4 Products

Product Documents for Human Nope/IGDCC4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Nope/IGDCC4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Nope/IGDCC4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10345

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Nope/IGDCC4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Nope/IGDCC4 Antibody

Detection of Human Nope/IGDCC4 by Western Blot.