Human NRG1 Isoform SMDF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF378
Key Product Details
Species Reactivity
Human
Applications
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NRG1 Isoform SMDF
Met1-Glu296
Accession # NP_039253
Met1-Glu296
Accession # NP_039253
Specificity
Detects human NRG1 Isoform SMDF in direct ELISAs and Western blots. Neutralizes 60‑80% of the biological activity of human NRG1 Isoform SMDF.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human NRG1 Isoform SMDF Antibody
Cell Proliferation Induced by NRG1 Isoform SMDF and Neutralization by Human NRG1 Isoform SMDF Anti-body.
Recombinant Human NRG1 Isoform SMDF (Catalog # 378-SM) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1 Isoform SMDF (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human NRG1 Isoform SMDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF378). The ND50 is typically 2-8 µg/mL.Applications for Human NRG1 Isoform SMDF Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human NRG1 Isoform SMDF (Catalog # 378-SM)
Sample: Recombinant Human NRG1 Isoform SMDF (Catalog # 378-SM)
Neutralization
Measured by its ability to neutralize NRG1 Isoform SMDF-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 2‑8 µg/mL in the presence of 12 ng/mL Recombinant Human NRG1 Isoform SMDF.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuregulin-1 Isoform SMDF
The heregulin (also known as neuregulin) family of cytokines is comprised of multiple secreted or membrane-bound isoforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. All heregulin family members share an epidermal growth factor (EGF)-like domain ( alpha- or beta‑variant) that interacts with the erbB family of tyrosine kinase receptors. NRG1 Isoform SMDF is a heregulin isoform containing a C-terminal EGF-like domain ( beta‑variant) and a unique N-terminal sequence that lacks an Ig-like domain which is present in all other known heregulins. NRG1 Isoform SMDF also lacks a transmembrane domain and the cytoplasmic tail. NRG1 Isoform SMDF expression has been found to be restricted to the nervous system. It is likely that NRG1 Isoform SMDF may play an important role in neural-specific functions.
References
- Yarden, Y. and D. Wen (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
- Meyer, D. et al. (1997) Development 124:3575.
Alternate Names
Neuregulin1 Isoform SMDF, SMDF
Gene Symbol
NRG1
UniProt
Additional Neuregulin-1 Isoform SMDF Products
Product Documents for Human NRG1 Isoform SMDF Antibody
Product Specific Notices for Human NRG1 Isoform SMDF Antibody
For research use only
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