Human Numb Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4338
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Numb
Thr356-Leu592
Accession # AAH68476
Thr356-Leu592
Accession # AAH68476
Specificity
Detects human Numb in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Numb Biotinylated Antibody
Numb in RT-4 Human Cell Line.
Numb was detected in immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line using Sheep Anti-Human Numb Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4338) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Numb Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line
Sample: Immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Numb
Sample: Recombinant Human Numb
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Numb
Alternate Names
m-numb, mnb, S171
Gene Symbol
NUMB
UniProt
Additional Numb Products
Product Documents for Human Numb Biotinylated Antibody
Product Specific Notices for Human Numb Biotinylated Antibody
For research use only
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