Human Numb Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4338
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Numb
Thr356-Leu592
Accession # AAH68476
Thr356-Leu592
Accession # AAH68476
Specificity
Detects human Numb in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Numb Antibody
Detection of Human Numb by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Numb Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4338) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Numb at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Numb in RT-4 Human Cell Line.
Numb was detected in immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line using Sheep Anti-Human Numb Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4338) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Numb Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed RT‑4 human urinary bladder transitional cell papilloma cell line
Sample: Immersion fixed RT‑4 human urinary bladder transitional cell papilloma cell line
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Numb
Alternate Names
m-numb, mnb, S171
Gene Symbol
NUMB
UniProt
Additional Numb Products
Product Documents for Human Numb Antibody
Product Specific Notices for Human Numb Antibody
For research use only
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