Human Orexin B/Hypocretin-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB734
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Camelid - Vicugna pacos (Alpaca)
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 145202
Product Specifications
Immunogen
KLH-conjugated human Orexin B/Hypocretin‑2 synthetic peptide
Accession # O43612
Accession # O43612
Specificity
Detects human Orexin B/Hypocretin‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Orexin A is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Orexin B/Hypocretin-2 Antibody
Orexin B in Mouse Hypothalamus.
Orexin B was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Mouse Anti-Human Orexin B Monoclonal Antibody (Catalog # MAB734) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the neuronal processes and synaptic boutons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem.
Orexin B, p38a, and Integrin beta1 were detected in perfusion fixed frozen sections of mouse brainstem using Mouse Anti-Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Anti-Mouse Integrin beta1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Human Orexin B/Hypocretin-2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brainstem and hypothalamus
Sample: Perfusion fixed frozen sections of mouse brainstem and hypothalamus
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Orexin B/Hypocretin-2
Alternate Names
Hcrt2, Hypocretin-2, Hypocretin2, PPORX, PPOX
Entrez Gene IDs
3060 (Human)
Gene Symbol
HCRT
UniProt
Additional Orexin B/Hypocretin-2 Products
Product Documents for Human Orexin B/Hypocretin-2 Antibody
Product Specific Notices for Human Orexin B/Hypocretin-2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...