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Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 456738

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Orexin R2/HCRTR2
Met1-Trp444
Accession # AAC39602

Specificity

Detects human Orexin R2/HCRTR2. Stains human Orexin R2/HCRTR2 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Orexin R2/HCRTR2 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: A172 human glioblastoma cell line and HEK293 human embryonic kidney cell line transfected with human Orexin R2/HCRTR2 and eGFP were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Orexin R2/HCRTR2

Hypocretin receptor 2 (HCRTR2); also known as orexin receptor 2 or OX2R is a 40 kDa 7-transmembrane G-protein-coupled glycoprotein that is a high affinity receptor for orexins A and B (hypocretins 1 and 2). In mouse brain, engagement of HCRTRs promotes wakefulness, such that absence of either orexins or their receptors creates a narcolepsy-like state. It also influences reward circuits involving food or addictive drugs. The extracellular portions of human HCRTR2 share 92% and 93% aa identity with corresponding portions of mouse and rat HCRTR2, respectively.

Long Name

Hypocretin Receptor Type 2

Alternate Names

HCRTR2, Orexin Receptor 2, OrexinR2, OX2R

Entrez Gene IDs

3062 (Human); 387285 (Mouse); 25605 (Rat)

Gene Symbol

HCRTR2

UniProt

Additional Orexin R2/HCRTR2 Products

Product Documents for Human Orexin R2/HCRTR2 Alexa Fluor® 700-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Orexin R2/HCRTR2 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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