HEK293 human embryonic kidney cell line transfected with human Orexin R2/HCRTR2 and eGFP was stained with either (A) Mouse Anti-Human Orexin R2/HCRTR2 Monoclonal Antibody (Catalog # MAB5246) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Orexin R2/HCRTR2 in A172 Human Cell Line by Flow Cytometry.

A172 human glioblastoma cell line was stained with Mouse Anti-Human Orexin R2/HCRTR2 Monoclonal Antibody (Catalog # MAB5246, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Orexin R2/HCRTR2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: A172 human glioblastoma cell line and HEK293 human embryonic kidney cell line transfected with human Orexin R2/HCRTR2 and eGFP were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Orexin R2/HCRTR2

Hypocretin receptor 2 (HCRTR2); also known as orexin receptor 2 or OX2R is a 40 kDa 7-transmembrane G-protein-coupled glycoprotein that is a high affinity receptor for orexins A and B (hypocretins 1 and 2). In mouse brain, engagement of HCRTRs promotes wakefulness, such that absence of either orexins or their receptors creates a narcolepsy-like state. It also influences reward circuits involving food or addictive drugs. The extracellular portions of human HCRTR2 share 92% and 93% aa identity with corresponding portions of mouse and rat HCRTR2, respectively.

Long Name

Hypocretin Receptor Type 2

Alternate Names

HCRTR2, Orexin Receptor 2, OrexinR2, OX2R

Entrez Gene IDs

3062 (Human); 387285 (Mouse); 25605 (Rat)

Gene Symbol

HCRTR2

UniProt

AAC39602

Additional Orexin R2/HCRTR2 Products

Product Documents for Human Orexin R2/HCRTR2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Orexin R2/HCRTR2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Orexin R2/HCRTR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5246

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Orexin R2/HCRTR2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Orexin R2/HCRTR2 Antibody

Detection of Orexin R2/HCRTR2 in HEK293 Human Cell Line Transfected with Human Orexin R2/HCRTR2 and eGFP by Flow Cytometry.