ORM2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

ORM2 in HepG2 Human Cell Line.

ORM2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of ORM2 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human ORM2 Antibody (Clone 959445)

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes and immersion fixed HepG2 human hepatocellular carcinoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: HepG2 human hepatocellular carcinoma cell line cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 1 review rated 5 using MAB9306 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ORM2

Orosomucoid 2 (ORM2) belongs to the family of lipocalin cytosolic fatty-acid binding proteins. ORM2 is a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. ORM2 may play a role in immunosuppression.

Long Name

Orosomucoid 2

Alternate Names

AGP-B, AGP2

Entrez Gene IDs

5005 (Human)

Gene Symbol

ORM2

UniProt

P19652

Additional ORM2 Products

Product Documents for Human ORM2 Antibody (Clone 959445)

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human ORM2 Antibody (Clone 959445)

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ORM2 Antibody (Clone 959445)

R&D Systems, part of Bio-Techne | Catalog # MAB9306

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ORM2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ORM2 Antibody (Clone 959445)

ORM2 in BG01V Human Embryonic Stem Cells.