Human Otx2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1979
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243
Met1-Leu289
Accession # P32243
Specificity
Detects human Otx2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Otx2 Biotinylated Antibody
Otx2 in BG01V Human Embryonic Stem Cells.
Otx2 was detected in immersion fixed BG01V human embryonic stem cells differentiated to ectoderm using Goat Anti-Human Otx2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated AntI-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Otx2 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line
Sample: Immersion fixed BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Otx2
Sample: Recombinant Human Otx2
Reviewed Applications
Read 1 review rated 4 using BAF1979 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Otx2
References
- Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.
Long Name
Orthodenticle Homolog 2
Alternate Names
homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2
Entrez Gene IDs
5015 (Human)
Gene Symbol
OTX2
UniProt
Additional Otx2 Products
Product Documents for Human Otx2 Biotinylated Antibody
Product Specific Notices for Human Otx2 Biotinylated Antibody
For research use only
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