Human OX40/TNFRSF4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10563
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Neutralization
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2553D
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived human OX40/TNFRSF4
Leu29-Ala216
Accession # P34389
Leu29-Ala216
Accession # P34389
Specificity
Detects human OX40/TNFRSF4 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human OX40/TNFRSF4 Antibody
Detection of OX40/TNFRSF4 in HEK293 Human Cell Line transfected with Human OX40/TNFRSF4 and eGFP by Flow Cytometry
HEK293 human embryonic kidney cell line transfected with either (A) human OX40/TNFRSF4 or (B) irrelevant protein, and eGFP, was stained with Rabbit anti-human OX40/TNFRSF4 monoclonal antibody (Catalog # MAB10563) followed by Allophycocyanin-conjugated anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on control antibody staining (MAB1050, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.CXCL8/IL-8 Secretion Induced by OX40L/TNFSF4 and Neutralization by Human OX40/TNFRSF4 Antibody.
Recombinant Human OX40L/TNFSF4 (1054-OX) stimulates CXCL8/IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40/TNFRSF4 in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (D8000C). CXCL8/Il-8 Secretion elicited by Recombinant Human OX40L/TNFSF4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human OX40/TNFRSF4 Monoclonal Antibody (Catalog # MAB10563). The ND50 is typically 25-250 ng/mL.Applications for Human OX40/TNFRSF4 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line transfected with Human OX40/TNFRSF4 and eGFP
Sample: HEK293 Human Cell Line transfected with Human OX40/TNFRSF4 and eGFP
Neutralization
Measured by its ability to neutralize OX40L/TNFSF4-induced IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40/TNFRSF4. The Neutralization Dose (ND50) is typically 25-250 ng/mL in the presence of 20 ng/mL Recombinant Human OX40L/TNFSF4.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OX40
References
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Alternate Names
ACT-135, CD134, Ly-70, TNFRSF4, Txgp1, TXGP1L
Gene Symbol
TNFRSF4
UniProt
Additional OX40 Products
Product Documents for Human OX40/TNFRSF4 Antibody
Product Specific Notices for Human OX40/TNFRSF4 Antibody
For research use only
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