Human P-Cadherin Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB861G
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Flow Cytometry, Knockout Validated
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 104805
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human P-Cadherin
Asp108-Gly654
Accession # CAA45177
Asp108-Gly654
Accession # CAA45177
Specificity
Detects human P-Cadherin in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) Cadherin‑8, recombinant mouse P-Cadherin, or rhVE-Cadherin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human P-Cadherin Alexa Fluor® 488-conjugated Antibody
Detection of P‑Cadherin in A431 Human Cell Line by Flow Cytometry.
A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human P-Cadherin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB861G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
P-Cadherin Specificity is Shown by Flow Cytometry in Knockout Cell Line.
P-Cadherin knockout A431 human epithelial carcinoma cell line was stained with Alexa Fluor® 488-conjugated Mouse Anti-Human P-Cadherin Monoclonal Antibody (Catalog # FAB861G, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the P-Cadherin knockout A431 cell line was observed. Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.Applications for Human P-Cadherin Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
5 µL/106 cells
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Knockout Validated
P-Cadherin is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in P-Cadherin knockout A431 cell line.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: P-Cadherin
Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.
References
- Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) Science 251:1451.
- Nose, A. et al. (1987) EMBO J. 6:3655.
Long Name
Placental Cadherin
Alternate Names
CAD3, Cadherin-3, CDH3, CDHP, HJMD, PCAD, PCadherin
Gene Symbol
CDH3
UniProt
Additional P-Cadherin Products
Product Specific Notices for Human P-Cadherin Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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