Human p53 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB13551
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 184727
Product Specifications
Immunogen
E. coli-derived recombinant human p53
Asp7-Asp393
Accession # P04637
Asp7-Asp393
Accession # P04637
Specificity
Detects human p53.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human p53 Antibody
Detection of p53 in MCF-7 cells by Flow Cytometry
MCF-7 cells were stained with Mouse Anti-Human p53 Monoclonal Antibody (Catalog # MAB13551, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human p53 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: MCF‑7 human breast cancer cell line fixed and permeabilized with FlowX FoxP3 Fixation and Permeabilization Buffer kit
Sample: MCF‑7 human breast cancer cell line fixed and permeabilized with FlowX FoxP3 Fixation and Permeabilization Buffer kit
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p53
The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
UniProt
Additional p53 Products
Product Documents for Human p53 Antibody
Product Specific Notices for Human p53 Antibody
For research use only
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