Human p53 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC13551A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 184727
Product Specifications
Immunogen
E. coli-derived recombinant human p53
Asp7-Asp393
Accession # P04637
Asp7-Asp393
Accession # P04637
Specificity
Detects human p53 in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human p53 APC-conjugated Antibody
Detection of p53 in MCF-7 cells by Flow Cytometry
MCF-7 cells were stained with Mouse Anti-Human p53 APC-conjugated Monoclonal Antibody (Catalog # IC13551A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human p53 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line fixed and permeabilized with FlowX FoxP3 Fixation and Permeabilization Buffer kit
Sample: MCF‑7 human breast cancer cell line fixed and permeabilized with FlowX FoxP3 Fixation and Permeabilization Buffer kit
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: p53
The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
UniProt
Additional p53 Products
Product Documents for Human p53 APC-conjugated Antibody
Product Specific Notices for Human p53 APC-conjugated Antibody
For research use only
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