Human PAK2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6849

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB6849
MAB6849-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 677411

View all PAK2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PAK2
Leu127-Gln221
Accession # Q13177

Specificity

Detects human PAK2 in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) PAK1 and no cross-reactivity with rhPAK1B, 3, 4, 6, or 7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human PAK2 Antibody

Detection of Human, Mouse, and Rat PAK2 antibody by Western Blot.

Detection of Human, Mouse, and Rat PAK2 by Western Blot.

Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PAK2 Monoclonal Antibody (Catalog # MAB6849) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PAK2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PAK2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HEL 92.1.7 human erythroleukemic cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAK2

PAK2 (p21-activated kinase-2) is a widely expressed 58-62 kDa cytoplasmic ser/thr kinase of the PAK family, with substrates including ribosomal S6 and histone H4 proteins. The 524 amino acid (aa) human PAK2 contains phosphorylation, acetylation (K38, K128) and myristoylation (G213) sites, a CRIB (cdc42/Rac interactive binding) domain (aa 74-87), a caspase-3 cleavage site after aa 212, and a kinase domain (aa 249-499). Full-length PAK2 stimulates cell growth and motility, while the caspase cleaved PAK2 kinase domain (34 kDa) is involved in cell death. Within the region used as an immunogen, human PAK2 shares 87% aa identity with mouse and rat PAK2. 

Long Name

p21-activated Kinase 2

Alternate Names

gamma-PAK, PAK65, S6/H4 Kinase

Entrez Gene IDs

5062 (Human); 224105 (Mouse); 29432 (Rat)

Gene Symbol

PAK2

UniProt

Q13177

Additional PAK2 Products

Product Documents for Human PAK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PAK2 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.