HT-29 human colon adeno-carcinoma cell line was stained with Mouse Anti-Human PAR2 PE-conjugated Mono-clonal Antibody (Catalog # FAB3949P, filled histogram) or isotype control antibody (IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of PAR2 in PC-3 cells by Flow Cytometry.

PC-3 cells were stained with Mouse Anti-Human PAR2 PE-conjugated Monoclonal Antibody (Catalog # FAB3949P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PAR2 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HT‑29 human colon adenocarcinoma cell line or PC-3 prostate cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: PAR2

Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.

Long Name

Protease-Activated Receptor 2

Alternate Names

CFIIRL1, F2RL1, GPCR11

Entrez Gene IDs

2150 (Human); 14063 (Mouse); 116677 (Rat)

Gene Symbol

F2RL1

UniProt

P55085

Additional PAR2 Products

Product Documents for Human PAR2 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PAR2 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human PAR2 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3949P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all PAR2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PAR2 PE-conjugated Antibody

Detection of PAR2 in HT‑29 Human Colon Adeno-carcinoma Cell Line by Flow Cytometry.