Human PAR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3949
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, N/A
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Binding Model, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 344222
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human PAR2
Accession # P55085
Accession # P55085
Specificity
Detects human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PAR2 Antibody
Detection of PAR2 in HT‑29 Human Cell Line by Flow Cytometry.
HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR2 Monoclonal Antibody (Catalog # MAB3949, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B).
Detection of PAR2 in PC-3 cells by Flow Cytometry.
PC-3 cells were stained with Mouse Anti-Human PAR2 Monoclonal Antibody (Catalog # MAB3949, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.Applications for Human PAR2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HT‑29 human colon adenocarcinoma cell line and PC-3 prostate cancer cell line
Sample: HT‑29 human colon adenocarcinoma cell line and PC-3 prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PAR2
Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.
Long Name
Protease-Activated Receptor 2
Alternate Names
CFIIRL1, F2RL1, GPCR11
Gene Symbol
F2RL1
UniProt
Additional PAR2 Products
Product Documents for Human PAR2 Antibody
Product Specific Notices for Human PAR2 Antibody
For research use only
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