Human Parkin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB14382
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 328110
Product Specifications
Immunogen
E. coli-derived recombinant human Parkin
Met1-Val437
Accession # NP_054642
Met1-Val437
Accession # NP_054642
Specificity
Detects human Parkin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Parkin Antibody
Parkin in Human Brain.
Parkin was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human Parkin Monoclonal Antibody (Catalog # MAB14382) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Parkin Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra)
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra)
Reviewed Applications
Read 1 review rated 5 using MAB14382 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Parkin
Parkin is a 465 amino acid protein with an N-terminal ubiquitin-like domain, a linker region, a C-terminal TRIAD domain consisting of two RING finger motifs flanking a cysteine-rich IBR (in between RING fingers) motif. Mutations in Parkin are a major cause of autosomal recessive juvenile parkinsonism. Parkin-2 lacks exon 5, which encodes amino acid residues 179‑206 of Parkin.
Long Name
Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1
Alternate Names
AR-JP, PARK2, PDJ, PRKN
Gene Symbol
PRKN
UniProt
Additional Parkin Products
Product Documents for Human Parkin Antibody
Product Specific Notices for Human Parkin Antibody
For research use only
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