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Human Pax5/BSAP Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC3487R

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1207C

Product Specifications

Immunogen

E. coli-derived recombinant human Pax5/BSAP
Thr141-His391
Accession # Q02548

Specificity

Detects human Pax5/BSAP in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Pax5/BSAP Alexa Fluor® 647-conjugated Antibody

Detection of Pax5/BSAP antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Pax5/BSAP in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Rabbit Anti-Human Pax5/BSAP Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC3487R) or (B) Normal Rabbit IgG Alexa Fluor® 647 Control (Catalog # IC1051R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human Pax5/BSAP Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pax5/BSAP

Pax5, also known as BSAP (B-cell-specific transcription factor) is a 44-48 kDa monomeric protein that belongs to the paired box transcription factor family of molecules. Human Pax5 is 391 amino acids (aa) in length and contains the paired DNA-binding domain over aa 16-142. More than 10 alternatively spliced isoforms with MW ranging from 25-40 kDa have been reported that likely possess different transactivation properties. Splicing may involve all but the first N-terminal 70 aa. Pax5 is principally found in pro-B cells and mature B cells where it promotes a B cell phenotype at the expense of plasma cell formation. In conjunction with Bcl-6, Pax5 represses XBP-1 and Blimp-1 expression. Over aa 141-391, human Pax5 shares 99% aa sequences identity with mouse Pax5.

Long Name

Paired Box Gene 5/B-cell Specific Activator Protein

Alternate Names

BSAP

Entrez Gene IDs

5079 (Human); 18507 (Mouse)

Gene Symbol

PAX5

UniProt

Additional Pax5/BSAP Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Pax5/BSAP Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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