Human/Mouse Pax5/BSAP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3487
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Multiplex Immunofluorescence, Simple Western, Western Blot
Cited:
Simple Western
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1207C
Product Specifications
Immunogen
E. coli-derived recombinant human Pax5
Thr141-His391
Accession # Q02548
Thr141-His391
Accession # Q02548
Specificity
Detects human Pax5 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse Pax5/BSAP Antibody
Detection of PAX5 in Human B-cell Lymphoma via seqIF™ staining on COMET™
PAX5 Antibody was detected in immersion fixed paraffin-embedded sections of human B-cell Lymphoma using Rabbit Anti-Human PAX5, Monoclonal Antibody (Catalog # MAB9857) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.
Detection of Human Pax5/BSAP by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Pax5/BSAP at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Pax5/BSAP in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) and either (A) Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) or (B) Normal Rabbit IgG Control (MAB1050) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.
Applications for Human/Mouse Pax5/BSAP Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Sample: Human peripheral blood mononuclear cells (PBMCs) fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed Daudi human Burkitt's lymphoma cell line (Positive) & Jurkat human acute T cell leukemia cell line (Negative)
Sample: Immersion fixed Daudi human Burkitt's lymphoma cell line (Positive) & Jurkat human acute T cell leukemia cell line (Negative)
Immunohistochemistry
3-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse embryo (13 d.p.c.)
Sample: Perfusion fixed frozen sections of mouse embryo (13 d.p.c.)
Multiplex Immunofluorescence
3 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human B-cell Lymphoma
Sample: Immersion fixed paraffin-embedded sections of Human B-cell Lymphoma
Simple Western
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm‑6 human Pre-B acute lymphocytic leukemia cell line
Sample: Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm‑6 human Pre-B acute lymphocytic leukemia cell line
Western Blot
0.1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm‑6 human Pre-B acute lymphocytic leukemia cell line
Sample: Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm‑6 human Pre-B acute lymphocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pax5/BSAP
Long Name
Paired Box Gene 5/B-cell Specific Activator Protein
Alternate Names
BSAP
Gene Symbol
PAX5
UniProt
Additional Pax5/BSAP Products
Product Documents for Human/Mouse Pax5/BSAP Antibody
Product Specific Notices for Human/Mouse Pax5/BSAP Antibody
For research use only
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