Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10348
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu124
Product Specifications
Immunogen
Human PD-L1/B7-H1
Specificity
Detects Human PD-L1/B7-H1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Atezolizumab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
Detection of PD-L1/B7-H1 in MDA-MB-231 human breast adenocarcinoma cell line by Flow Cytometry.
MDA-MB-231 human breast adenocarcinoma cell line was stained with Human Anti-Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10348, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (F0135). View our protocol for Staining Membrane-associated Proteins.
PD-1 binding to Human PD-L1/B7-H1-transfected HEK293 Human Cell Line is Blocked by Human PD-L1/B7-H1 Antibody.
In a functional flow cytometry test, Recombinant Human PD-1 His-tagged protein (8986-PD, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 2.5 µg/mL of Human Anti-Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10348) but not by (B) Human IgG Isotype Control (1-001-A). Ligand binding was detected by staining cells with Anti-His tag APC-conjugated Monoclonal Antibody (IC050A).Applications for Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional flow cytometry test, 2.5 μg/mL of Human anti-human PD-L1/B7-H1 (Atezoliumab Biosimilar) Antibody(Catalog # MAB10348) will block the binding of Recombinant Human PD-1 His-tagged protein (Catalog # 8986-PD) to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: MDA-MB-231 human breast adenocarcinoma cell line
Sample: MDA-MB-231 human breast adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-L1/B7-H1
Long Name
Programmed Death Ligand 1
Alternate Names
B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1
Entrez Gene IDs
Gene Symbol
CD274
Additional PD-L1/B7-H1 Products
Product Documents for Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
Product Specific Notices for Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
For research use only
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