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Human PEDFR/PNPLA2 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5387G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 494702

Product Specifications

Immunogen

E. coli-derived recombinant human PEDF R/PNPLA2
Val162-Thr332
Accession # Q96AD5

Specificity

Detects human PEDF R/PNPLA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse PEDF R is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PEDFR/PNPLA2 Alexa Fluor® 488-conjugated Antibody

Detection of PEDF R/PNPLA2 antibody in Y-79 Human Cell Line antibody by Flow Cytometry.

Detection of PEDF R/PNPLA2 in Y‑79 Human Cell Line by Flow Cytometry.

Y-79 human retinoblastoma cell line was stained with Mouse Anti-Human PEDF R/PNPLA2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB5387G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PEDFR/PNPLA2 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Y‑79 human retinoblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PEDFR/PNPLA2

PEDF R (Pigment Epithelium-derived Factor Receptor), also known as PNPLA2, ATGL, TTS2.2, iPLA2 zeta (Calcium-independent Phospholipase A2 zeta) and Desnutrin, is a 82-86 kDa type II transmembrane protein that is a member of the patatin-like phospholipase domain-containing protein family (gene name PNPLA2). PEDF is highly expressed in adipose tissue (both white and brown fat cells), where it the catalyzes formation of diacyglycerol from triglyceride, the transfer of an acyl (CoA-fatty acid) derivative to acyl-glycerol, and releases arachidonic acid from intracellular lipid deposits. It has also been found on retinal pigment epithelium, endothelial cells, hepatocytes, cerebellar granule cells, macrophages, mast cells, ovarian granulosa cells, retinal ganglia neurons and skeletal muscle cells. PEDF R is directly activated by PEDF, and indirectly by  beta3-AR which promotes CGI-58 association. Human PEDF R shares 85% amino acid (aa) sequence identity with mouse and rat PEDF R within aa 162‑332, the region used as an immunogen.

Long Name

Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2

Alternate Names

ATGL, Desnutrin, IPLA2-zeta, PEDF R, PNPLA2, TTS2

Entrez Gene IDs

57104 (Human); 66853 (Mouse); 361676 (Rat)

Gene Symbol

PNPLA2

UniProt

Additional PEDFR/PNPLA2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PEDFR/PNPLA2 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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