Human Peroxiredoxin 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3488
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 477609
Product Specifications
Immunogen
E. coli-derived recombinant human Peroxiredoxin 1
Met1-Lys199
Accession # Q06830
Met1-Lys199
Accession # Q06830
Specificity
Detects endogenous human Peroxiredoxin 1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Peroxiredoxin 1 Antibody
Detection of Human Peroxiredoxin 1 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Peroxiredoxin 1 in MCF-7 Human Cell Line.
Peroxiredoxin 1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Detection of Human Peroxiredoxin 1 by Simple WesternTM.
Simple Western shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Peroxiredoxin 1 at approximately 28 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.Applications for Human Peroxiredoxin 1 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Immunoprecipitation
1 µg/1 mg cell lysate
Sample: Cell lysate of U2OS human osteosarcoma cell line
Sample: Cell lysate of U2OS human osteosarcoma cell line
Knockout Validated
Peroxiredoxin 1 is specifically detected in the parental U2OS cell line, but is not detectable in knockout U2OS cell line.
Simple Western
10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Western Blot
0.1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line
Sample: Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line
Reviewed Applications
Read 1 review rated 5 using MAB3488 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 1
Alternate Names
NKEF-A, PRDX1, PRX1, TDPX2
Gene Symbol
PRDX1
UniProt
Additional Peroxiredoxin 1 Products
Product Documents for Human Peroxiredoxin 1 Antibody
Product Specific Notices for Human Peroxiredoxin 1 Antibody
For research use only
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