Human Phospho-Bad (S99) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7764
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 679431
Product Specifications
Immunogen
Phosphopeptide containing the human Bad S99 site
Accession # Q92934
Accession # Q92934
Specificity
Detects human Bad when phosphorylated at S99 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Phospho-Bad (S99) Antibody
Phospho-Bad (S99) in HEK293 Human Cell Line.
Bcl-xL/Bcl-2 Associated Death Promoter phosphorylated at S99 (Phospho-Bad (S99)) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human Phospho-Bad (S99) Monoclonal Antibody (Catalog # MAB7764) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Note that labeling is absent in dividing cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospho-Bad (S99) Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line
Sample: Immersion fixed HEK293 human embryonic kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bad
Bcl-2 antagonist of cell death (Bad) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser75, Ser99, and Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Phosphorylation of Ser75 and Ser99 is also required for the ability of Bad to induce cell cycle arrrest in G1. Human Bad shares 75 % aa sequence identity with mouse and rat Bad.
Long Name
Bcl-xL/Bcl-2 Associated Death Promoter
Alternate Names
BBC6, BCL2L8
Gene Symbol
BAD
UniProt
Additional Bad Products
Product Documents for Human Phospho-Bad (S99) Antibody
Product Specific Notices for Human Phospho-Bad (S99) Antibody
For research use only
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