Human Phospho-BTK (Y223) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7205
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 720101
Product Specifications
Immunogen
Phosphopeptide containing the human BTK Y223 site
Specificity
Detects human Phospho-BTK (Y223) in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Phospho-BTK (Y223) Antibody
Detection of Human Phospho-BTK (Y223) by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate for 30 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-BTK (Y223) Monoclonal Antibody (Catalog # MAB7205) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-BTK (Y223) at approximately 80 kDa (as indicated). For additional reference, duplicate samples were probed with 0.05 µg/mL of Mouse Anti-Human BTK Monoclonal Antibody (lower panel, Catalog # MAB5807) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-BTK (Y223) in Raji Human Cell Line.
BTK phosphorylated at Y223 was detected in immersion fixed Raji human Burkitt's lymphoma cell line with (upper panel) and without (lower panel) pervanadate stimulation using Mouse Anti-Human Phospho-BTK (Y223) Monoclonal Antibody (Catalog # MAB7205) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Phospho-BTK (Y223) Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line with and without pervanadate stimulation
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line with and without pervanadate stimulation
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line treated with Pervanadate
Sample: Raji human Burkitt's lymphoma cell line treated with Pervanadate
Reviewed Applications
Read 2 reviews rated 5 using MAB7205 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTK
Long Name
Bruton Agammaglobulinemia Tyrosine Kinase
Alternate Names
AGMX1, IMD1, PSCTK1, XLA
Entrez Gene IDs
695 (Human)
Gene Symbol
BTK
Additional BTK Products
Product Documents for Human Phospho-BTK (Y223) Antibody
Product Specific Notices for Human Phospho-BTK (Y223) Antibody
For research use only
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