Human Phospho-Caveolin-1 (Y14) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7418
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 750304
Product Specifications
Immunogen
Phosphopeptide containing the human Caveolin-1 Y14 site
Accession # Q03135
Accession # Q03135
Specificity
Detects human Phospho-Caveolin-1 (Y14) in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Phospho-Caveolin-1 (Y14) Antibody
Detection of Human Phospho-Caveolin‑1 (Y14) by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-Caveolin-1 (Y14) Monoclonal Antibody (Catalog # MAB7418) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Caveolin-1 (Y14) at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-Caveolin-1 (Y14) in MCF‑7 Human Cell Line.
Caveolin-1 phosphorylated at Y14 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Phospho-Caveolin-1 (Y14) Monoclonal Antibody (Catalog # MAB7418) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospho-Caveolin-1 (Y14) Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Western Blot
0.2 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line treated with Pervanadate
Sample: HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line treated with Pervanadate
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caveolin-1
Alternate Names
CAV1, Caveolin1, MSTP085, VIP21
Gene Symbol
CAV1
UniProt
Additional Caveolin-1 Products
Product Documents for Human Phospho-Caveolin-1 (Y14) Antibody
Product Specific Notices for Human Phospho-Caveolin-1 (Y14) Antibody
For research use only
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