Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-EGFR (Y1068) Monoclonal Antibody (Catalog # MAB3570), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-EGFR (Y1068) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Phospho-EGFR in EGF-treated A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was untreated (open histogram) or treated for 5 minutes with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG, filled histogram) then stained with Human Phospho--EGFR (Y1068) Monoclonal Antibody (Catalog # MAB3570), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). Mouse IgG_2A(Catalog # MAB003, data not shown) was used as an isotype control. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human Phospho-EGFR (Y1068) Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG), fixed with paraformaldehyde, and permeabilized with saponin

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB3570 in the following applications:

Microarray (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

Epidermal growth factor receptor (EGFR, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation at Tyr 1068 allows binding of the SH2 domain of the cytosolic adaptor Grb2. This binding results in Ras activation.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

Additional EGFR Products

Product Documents for Human Phospho-EGFR (Y1068) Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human Phospho-EGFR (Y1068) Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Phospho-EGFR (Y1068) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3570

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all EGFR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-EGFR (Y1068) Antibody

Detection of Human Phospho-EGFR (Y1068) by Western Blot.