A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG) was stained with Mouse Anti-Human Phospho-EGF R/ErbB1 (Y1068) Fluorescein-conjugated Monoclonal Antibody (Catalog # IC3570F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG) and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: EGFR

Epidermal growth factor receptor (EGF R, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation at Tyr 1068 allows binding of the SH2 domain of the cytosolic adaptor Grb2. This binding results in Ras activation.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

Additional EGFR Products

Product Documents for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC3570F

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all EGFR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry.