Human Phospho-eIF2 alpha (S52) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB39971
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 849159
Product Specifications
Immunogen
Phosphopeptide containing the human eIF2 alpha S52 site
Accession # P05198
Accession # P05198
Specificity
Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human Phospho-eIF2 alpha (S52) Antibody
Detection of Human Phospho-eIF2 alpha (S52) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes or 20 mJ/cm2 ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-eIF2a (S52) Monoclonal Antibody (Catalog # MAB39971) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-eIF2a (S52) at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-eIF2 alpha (S52) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA) or ultraviolet light (UV)
Sample: HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA) or ultraviolet light (UV)
Reviewed Applications
Read 1 review rated 5 using MAB39971 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eIF2 alpha
Long Name
Eukaryotic Translation Initiation Factor 2 Alpha
Alternate Names
EIF2S1
Gene Symbol
EIF2S1
UniProt
Additional eIF2 alpha Products
Product Documents for Human Phospho-eIF2 alpha (S52) Antibody
Product Specific Notices for Human Phospho-eIF2 alpha (S52) Antibody
For research use only
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