Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1825
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 798508
Product Specifications
Immunogen
Phosphopeptide containing the ERK1 T202/Y204 site
Specificity
Detects human ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody
Detection of Human and Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 5 minutes and 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB1825) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-ERK1 (T202/Y204) and Phospho-ERK2 (T185/Y187) at approximately 43 and 41 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human PDGF‑BB (Catalog # 220-BB) or PMA
Sample: NIH‑3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human PDGF‑BB (Catalog # 220-BB) or PMA
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERK1/ERK2
Long Name
Extracellular Signal-regulated Kinase 1/2
Alternate Names
ERK1, ERK-1, ERT2, Extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAPK 1, Microtubule-associated protein 2 kinase, mitogen-activated protein kinase 3, p44erk1, p44-ERK1, p44mapk, p44-MAPK, PRKM3
Additional ERK1/ERK2 Products
Product Documents for Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody
Product Specific Notices for Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody
For research use only
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