Recombinant Human PDGF-BB Protein, CF Best Seller
R&D Systems, part of Bio-Techne | Catalog # 220-BB
Key Product Details
Source
E. coli
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
E. coli-derived human PDGF-BB protein
Ser82-Thr190
Ser82-Thr190
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser82
Predicted Molecular Mass
12.3 kDa (monomer)
SDS-PAGE
12 kDa, reducing conditions
Activity
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 1.5-6 ng/mL.
The ED50 for this effect is 1.5-6 ng/mL.
Reviewed Applications
Read 13 reviews rated 4.4 using 220-BB in the following applications:
Scientific Data Images for Recombinant Human PDGF-BB Protein, CF
Recombinant Human PDGF-BB Protein Bioactivity
Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.Recombinant Human PDGF-BB Protein SDS-PAGE
1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.Formulation, Preparation and Storage
220-BB
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile 4 mM HCl.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: PDGF-BB
Long Name
Platelet-derived Growth Factor BB
Alternate Names
PDGFBB
Gene Symbol
PDGFB
UniProt
Additional PDGF-BB Products
Product Documents for Recombinant Human PDGF-BB Protein, CF
Product Specific Notices for Recombinant Human PDGF-BB Protein, CF
For research use only
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