Recombinant Human PDGF-BB Protein, CF Best Seller

R&D Systems, part of Bio-Techne | Catalog # 220-BB

R&D Systems, part of Bio-Techne
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220-BB-010
220-BB-01M
220-BB-050
220-BB-200
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Key Product Details

Source

E. coli

Accession #

P01127.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PDGF-BB Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human PDGF-BB protein
Ser82-Thr190

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser82

Predicted Molecular Mass

12.3 kDa (monomer)

SDS-PAGE

12 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 1.5-6 ng/mL.

Reviewed Applications

Read 12 reviews rated 4.5 using 220-BB in the following applications:

Scientific Data Images for Recombinant Human PDGF-BB Protein, CF

Recombinant Human PDGF-BB Protein Bioactivity

Recombinant Human PDGF-BB Protein Bioactivity

Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.
Recombinant Human PDGF-BB Protein SDS-PAGE

Recombinant Human PDGF-BB Protein SDS-PAGE

1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.

Formulation, Preparation and Storage

220-BB
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name

Platelet-derived Growth Factor BB

Alternate Names

PDGFBB

Entrez Gene IDs

5155 (Human); 24628 (Rat)

Gene Symbol

PDGFB

UniProt

P01127.1

Additional PDGF-BB Products

Product Documents for Recombinant Human PDGF-BB Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PDGF-BB Protein, CF

For research use only

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