Human Phospho-Lck (Y505) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7369
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 755216
Product Specifications
Immunogen
Phosphopeptide containing the human Lck (Y505) site.
Accession # P06239
Accession # P06239
Specificity
Detects human Phospho-Lck (Y505) in direct ELISA and Western Blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Phospho-Lck (Y505) Antibody
Detection of Human Phospho-Lck (Y505) by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 10 minutes or 2mM H2O2for 2 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-Lck (Y505) Monoclonal Antibody (Catalog # MAB7369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Lck (Y505) at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-Lck (Y505) in Jurkat Human Cell Line.
Lck phosphorylated at Y505 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated (upper panel) or not (lower panel) with pervanadate using Mouse Anti-Human Phospho-Lck (Y505) Monoclonal Antibody (Catalog # MAB7369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Phospho-Lck (Y505) Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line stimulated with pervanadate
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line stimulated with pervanadate
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate or H2O2
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate or H2O2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lck
Lck (p56lck) is a 56 kDa cytosolic phosphoprotein within the Src family of non-receptor tyrosine kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121), an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). Within aa 2-66, human Lck shares 89% aa sequence identity with mouse and rat Lck. A short (363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4 and CD8 molecules and plays a pivotal role in regulating T cell activation. It can be overexpressed in cancers and functions as an oncogene.
Long Name
Lymphocyte Protein Tyrosine Kinase
Alternate Names
Hck-3, Lcktkr, LSTRA, p56lck, pp58lck, YT16
Gene Symbol
LCK
UniProt
Additional Lck Products
Product Documents for Human Phospho-Lck (Y505) Antibody
Product Specific Notices for Human Phospho-Lck (Y505) Antibody
For research use only
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