Human Phospho-PLC-gamma 2 (Y759) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7377
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 744757
Product Specifications
Immunogen
Phosphopeptide containing the human PLC-gamma 2 Y759 site
Accession # P16885
Accession # P16885
Specificity
Detects human Phospho-PLC-gamma 2 (Y759) in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Phospho-PLC-gamma 2 (Y759) Antibody
Detection of Human Phospho-PLC‑ gamma2 (Y759) by Western Blot.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes or 10 µg/mL Goat Anti-Human IgM µ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C) for 2 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-PLC- gamma2 (Y759) Monoclonal Antibody (Catalog # MAB7377) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-PLC- gamma2 (Y759) at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-PLC‑ gamma2 (Y759) in Ramos Human Cell Line.
PLC- gamma2 phosphorylated at Y759 was detected in immersion fixed Ramos human Burkitt's lymphoma cell line treated (upper panel) or untreated (lower panel) with pervanadate for 5 minutes using Mouse Anti-Human Phospho-PLC- gamma2 (Y759) Monoclonal Antibody (Catalog # MAB7377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Phospho-PLC-gamma 2 (Y759) Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Ramos human Burkitt's lymphoma cell line treated with pervanadate (PV)
Sample: Immersion fixed Ramos human Burkitt's lymphoma cell line treated with pervanadate (PV)
Western Blot
0.2 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line treated with Pervanadate (PV) or Goat Anti-Human IgM μ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C)
Sample: Ramos human Burkitt's lymphoma cell line treated with Pervanadate (PV) or Goat Anti-Human IgM μ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-gamma 2
The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-delta, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones, and downstream through Lck kinase-dependent phosphorylation at Y753 and Y759. Activated PLC-gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes. Human PLC-gamma 2 shares 94% and 95% sequence identity overall with mouse and rat PLC-gamma 2, respectively, and 100% sequence identity within the peptide immunogen.
Long Name
Phospholipase C gamma 2
Alternate Names
PLCG2, PLCgamma 2
Gene Symbol
PLCG2
UniProt
Additional PLC-gamma 2 Products
Product Documents for Human Phospho-PLC-gamma 2 (Y759) Antibody
Product Specific Notices for Human Phospho-PLC-gamma 2 (Y759) Antibody
For research use only
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