Human Phospho-RelA/NFkB p65 (S536) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB72261
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1091B
Product Specifications
Immunogen
Phosphopeptide containing the RelA/NF kappaB p65 S536 site
Specificity
Detects human RelA/NF kappaB p65 when phosphorylated at S536.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-RelA/NFkB p65 (S536) Antibody
Detection of Human Phospho-RelA/NF kappaB p65 (S536) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB72261) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RelA/NF kappa B p65 (S536) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-RelA/NF kappaB p65 (S536) by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-RelA/NF kappa B p65 (S536) at approximately 66 kDa (as indicated) using 1.0 µg/mL of Rabbit Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB72261). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Phospho-RelA/NFkB p65 (S536) in HT‑29 Human Cell Line.
RelA/NF kappa B p65 phosphorylated at S536 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line untreated (lower panel) or treated with Calyculin A (Catalog # 1336) and Recombinant Human TNF-a (Catalog # 210-TA; upper panel) using Rabbit Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB72261) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospho-RelA/NFkB p65 (S536) Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line untreated or treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line untreated or treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Simple Western
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Sample: HeLa human cervical epithelial carcinoma cell line treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Western Blot
0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Sample: HeLa human cervical epithelial carcinoma cell line treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RelA/NFkB p65
Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog A
Alternate Names
NFkB p65, NFKB3, p65RelA
Entrez Gene IDs
5970 (Human)
Gene Symbol
RELA
Additional RelA/NFkB p65 Products
Product Documents for Human Phospho-RelA/NFkB p65 (S536) Antibody
Product Specific Notices for Human Phospho-RelA/NFkB p65 (S536) Antibody
For research use only
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