Human Phospholipid Scramblase 1/PLSCR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8137
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 875327
Product Specifications
Immunogen
E. coli-derived recombinant human Phospholipid Scramblase 1/PLSCR1
Met1-Pro84
Accession # O15162
Met1-Pro84
Accession # O15162
Specificity
Detects human Phospholipid Scramblase 1/PLSCR1 in ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Phospholipid Scramblase 1/PLSCR1 Antibody
Detection of Human Phospholipid Scramblase 1/PLSCR1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospholipid Scramblase 1/PLSCR1 Monoclonal Antibody (Catalog # MAB8137) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospholipid Scramblase 1/PLSCR1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospholipid Scramblase 1/PLSCR1 in HT‑29 Human Cell Line.
Phospholipid Scramblase 1/PLSCR1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human Phospholipid Scramblase 1/PLSCR1 Monoclonal Antibody (Catalog # MAB8137) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospholipid Scramblase 1/PLSCR1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Phospholipid Scramblase 1/PLSCR1
Alternate Names
MMTRA1B, PLSCR1
Gene Symbol
PLSCR1
UniProt
Additional Phospholipid Scramblase 1/PLSCR1 Products
Product Documents for Human Phospholipid Scramblase 1/PLSCR1 Antibody
Product Specific Notices for Human Phospholipid Scramblase 1/PLSCR1 Antibody
For research use only
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