Human PIAS2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4768
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PIAS2
Met1-Gly550
Accession # O75928
Met1-Gly550
Accession # O75928
Specificity
Detects human PIAS2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PIAS2 Antibody
Detection of Human PIAS2 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PIAS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PIAS2 at approximately 63-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PIAS2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line
Sample: MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIAS2
Long Name
Protein Inhibitor of Activated STAT 2
Alternate Names
ARIP3, DIP, MIZ1, PIASx alpha, SIZ2, ZMIZ4
Gene Symbol
PIAS2
UniProt
Additional PIAS2 Products
Product Documents for Human PIAS2 Antibody
Product Specific Notices for Human PIAS2 Antibody
For research use only
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