Human PIAS2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4768

R&D Systems, part of Bio-Techne
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AF4768
AF4768-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all PIAS2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PIAS2
Met1-Gly550
Accession # O75928

Specificity

Detects human PIAS2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human PIAS2 Antibody

Detection of Human PIAS2 antibody by Western Blot.

Detection of Human PIAS2 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PIAS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PIAS2 at approximately 63-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PIAS2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIAS2

PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx-beta) is a 45‑70 kDa member of the PIAS family of molecules. In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis. However, it is widely expressed in cultured cell lines. PIAS2 is known to inhibit STAT4-mediated gene activation, and to interact with inactive ELK‑1, promoting its activation via HDAC2 dissociation and desumoylation. Human PIAS2 is 621 amino acids (aa) in length. It contains a SAP domain (aa 11‑45), a zinc-finger region (aa 331‑408), a SUMO-binding motif (aa 467‑472) and an NLS (aa 484‑492). There are multiple splice variants. PIAS2x-beta /ARIP3 shows a 22 aa substitution for aa 551‑621, while PIAS-NY shows a 12 aa substitution for aa 1‑8, followed by a truncation after Gly401. Two others show a four aa substitution for aa 504‑621, and an alternate start site at Met10. Over aa 1‑550, human PIAS2 shares 98% aa identity with mouse PIAS2.

Long Name

Protein Inhibitor of Activated STAT 2

Alternate Names

ARIP3, DIP, MIZ1, PIASx alpha, SIZ2, ZMIZ4

Entrez Gene IDs

9063 (Human); 17344 (Mouse)

Gene Symbol

PIAS2

UniProt

O75928

Additional PIAS2 Products

Product Documents for Human PIAS2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PIAS2 Antibody

For research use only

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