Human PIWIL1/HIWI Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6548
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 585509
Product Specifications
Immunogen
E. coli-derived recombinant human PIWIL1/HIWI
Arg82-Thr290
Accession # NP_004755
Arg82-Thr290
Accession # NP_004755
Specificity
Detects human PIWIL1/HIWI in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) PIWIL2 or rhPIWIL4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PIWIL1/HIWI Antibody
PIWIL1/HIWI in NCI‑N87 Human Cell Line.
PIWIL1/HIWI was detected in immersion fixed NCI-N87 human gastric carcinoma cell line using Mouse Anti-Human PIWIL1/HIWI Monoclonal Antibody (Catalog # MAB6548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm in colonies of NCI-N87 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PIWIL1/HIWI Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed NCI-N87 human gastric carcinoma cell line
Sample: Immersion fixed NCI-N87 human gastric carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIWIL1/HIWI
HIWI (human homolog of drosophila piwi), also called PIWIL1 (piwi-like 1) is an 861 amino acid, 99 kDa (predicted) cytoplasmic protein of the PIWI subgroup of the Argonaute family. PIWI domains bind a class of ~29-30 nucleotide RNAs termed piRNAs (piwi domain-binding RNAs). PIWIL1 orthologs are expressed with piRNAs in germ cells and are essential for spermatogenesis in mice. CD34+ hematopoietic progenitor cell PIWIL1 is downregulated upon differentiation. When overexpressed in leukemic cell lines, PIWIL1 inhibits growth and induces apoptosis. Human PIWIL1 shares 95% amino acid sequence identity with mouse and rat PIWIL1.
Long Name
Piwi-like Protein 1
Alternate Names
HIWI, MIWI
Entrez Gene IDs
9271 (Human)
Gene Symbol
PIWIL1
UniProt
Additional PIWIL1/HIWI Products
Product Documents for Human PIWIL1/HIWI Antibody
Product Specific Notices for Human PIWIL1/HIWI Antibody
For research use only
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