Human PLA2G16 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6190
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PLA2G16
Asp12-Asp132
Accession # P53816
Asp12-Asp132
Accession # P53816
Specificity
Detects human PLA2G16 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PLA2G16 Antibody
Detection of Human PLA2G16 by Western Blot.
Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PLA2G16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6190) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLA2G16 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human PLA2G16 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLA2G16
PLA2G16 (Group 16 phospholipase A2; also HREV107 and AdPLA) is a Ca++‑dependent 17‑18 kDa member of the H‑rev107 family of molecules. It is expressed intracellularly by white adipocytes, induced by insulin, and generates arachadonic acid from phospholipids. The arachadonic acid is converted to PGE2, which binds the EP3 receptor and decreases cAMP levels. This results in decreased hormone‑sensitive lipase activity and increased adiposity. Human PLA216 is 162 amino acids (aa) in length. It is associated with both membranes and cytosol, and may possess a transmembrane segment between aa 134‑154. The enzymatic activity resides in His23 and Cys113. There is one potential isoform that shows a 20 aa substitution for the first four amino acids. Over aa 12 ‑ 132, human PLA2G16 shares 88% aa identity with mouse PLA2G16.
Long Name
Phospholipase A2, Group XVI
Alternate Names
AdPLA, HRASLS3, HREV107
Gene Symbol
PLAAT3
UniProt
Additional PLA2G16 Products
Product Documents for Human PLA2G16 Antibody
Product Specific Notices for Human PLA2G16 Antibody
For research use only
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