Human Plexin A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB65361
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 708954
Product Specifications
Immunogen
E. coli-derived recombinant human Plexin A1
Tyr964-Leu1152
Accession # Q9UIW2
Tyr964-Leu1152
Accession # Q9UIW2
Specificity
Detects human Plexin A1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Plexin A1 Antibody
Detection of Plexin A1 in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB65361, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Goat anti-Mouse IgG secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Detection of Plexin A1 in MDA-MB-231 cells and Daudi cells.
Plexin A1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line (Positive) and absent in Daudi human Burkitt's lymphoma cell line (Negative) using Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB65361) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Plexin A1 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line and Daudi human Burkitt's lymphoma cell line.
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line and Daudi human Burkitt's lymphoma cell line.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin A1
Plexin A1 is an approximately 200 kDa type I transmembrane member of the Plexin family of Semaphorin signal transducers. It consists of a 1218 amino acid (aa) extracellular domain with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain. Plexin A1 binds the transmembrane Semaphorin 6D directly and binds the Class 3 secreted Semaphorins indirectly via Neuropilin-1 and -2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. Within aa 964-1152 (in the IPT/TIG region), human Plexin A1 shares 93% aa sequence identity with mouse and rat Plexin A1.
Alternate Names
NOVP, PLXN1, PLXNA1
Gene Symbol
PLXNA1
UniProt
Additional Plexin A1 Products
Product Documents for Human Plexin A1 Antibody
Product Specific Notices for Human Plexin A1 Antibody
For research use only
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